Thalidomide antagonized the formation SNP induced stress fibers in ECs. A SNP (500 μmol) treatment induced stress fiber formation in ECs by two and a half fold in comparison to that of DMSO treated (control) cells. When SNP treated cells were challenged with 25, 50 and 75 μg/ml thalidomide respectively, cells from the 50 and 75 μg/ml thalidomide groups showed 30% and 50% drops in the number of stress fibers respectively in comparison to that of only SNP treated cells. SNP, Tx25, Tx50 and Tx75 denote 500 μmol SNP, 25, 50 and 75 μg/ml thalidomide respectively. The asterisks represent the level of significance. * P < 0.05 compared to only SNP treated cells.