Figure 9
From: Thalidomide attenuates nitric oxide mediated angiogenesis by blocking migration of endothelial cells
Thalidomide attenuates DEAN induced tube formation in ECs. ECs were seeded on collagen plated 12 well plates with 60% cell density, to form monolayers after 24 hours of incubation at 37°C/5% CO2. Total number of DEAN (10 μmol) induced tubes were counted after 12 hours of incubation with thalidomide (25, 50, and 75 μgm/ml). * P < 0.05 compared to only DEAN treated cells.