Figure 3

The expression of hTERT, c-myc, PPARγ, cyclin D and bcl-2 genes over 12 h (hours) in normal human PBMCs treated with growth medium containing 10% NHS and 2.5 mM EGTA. The cells were harvested at indicated time points and subjected to RT-PCR and western blotting. (A) Representative agarose gel photographs showing ethidium bromide stained RT-PCR products of hTERT, c-myc, PPARγ and β₂M genes. (B) Graph showing the changes in mRNA levels of hTERT, c-myc and PPARγ genes with increasing time after EGTA treatment. The relative levels of hTERT, c-myc and PPARγ mRNA expression were determined by measuring their band intensities using SCION IMAGE analysis software and normalizing to β₂M band intensity. Data are expressed as percentage of control (0 h). Each data point represents mean ± SD of three independent experiments. (C) Western blot analysis of expression of cyclin D and bcl-2 proteins. Equal amount of protein was used for the four samples. The western blot shown is a representative of three independent experiments.