Pim-1 colocalizes with Runx-1 and Runx-3. The subcellular distribution of ECFP-Pim-1, EYFP-Runx1 and EYFP-Runx3 was analysed from transiently transfected COS-7 cells under confocal microscope. Shown are single- (A) or double-positive (B, C) cells expressing indicated fluorescent proteins. Colocalization of ECFP (first panel) and EYFP (second panel) fusion proteins in the circled nuclei was visualized by yellow colour in merged images (third panel) and was confirmed by scattergram plots (fourth panel), where the intensities of the CFP and YFP channels are on the X- and Y-axis, respectively. Bar represents 20 μm.