Figure 5
Effect of SFLLR-NH2, an agonist peptide of PAR-1 and its reverse peptide RLLFS-NH2 on IL-8 release from A549 cells. Cells were incubated (A) with various concentrations of SFLLR-NH2 (open bar) or RLLFS-NH2 (hatched bar) at 37°C for 16 h or (B) with 100 μM of SFLLR-NH2 and RLLFS-NH2 for 2 h, 8 h and 16 h. Values shown are Mean ± SE for five separate experiments performed in duplicate. *P < 0.05 compared with the response to medium alone control; †P < 0.05 compared with the response to RLLFS-NH2 at the same concentration.