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Table 2 Effect of hirudin on IL-8 release from A549 cells induced by thrombin

From: Induction of release and up-regulated gene expression of interleukin (IL)-8 in A549 cells by serine proteinases

Compound (U/ml) IL-8 released (pg/ml)
  2 h 16 h
Medium alone 18.7 ± 1.6 64.8 ± 7.2
Thrombin 3 40.1 ± 11.1 181 ± 32
Hirudin 3 18.4 ± 1.7 52.8 ± 5.7
Thrombin 3 + Hirudin 3 21.9 ± 7.2* 73.9 ± 12.9*
Thrombin 10 65.3 ± 18.4 478 ± 131
Hirudin 10 18.9 ± 2.1 59.3 ± 12.6
Thrombin 10 + Hirudin 10 26.8 ± 6.7* 119 ± 16.6*
  1. Data shown are mean ± SE for five separate experiments. Thrombin, hirudin, and thrombin with hirudin were added to cells for 2 h or 16 h. *P < 0.05 compared with the corresponding concentration of thrombin alone.