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Table 3 Effect of trypsin inhibitors on IL-8 release from A549 cells induced by trypsin

From: Induction of release and up-regulated gene expression of interleukin (IL)-8 in A549 cells by serine proteinases

Compound (μg/ml) IL-8 released (pg/ml)
  8 h 16 h
Medium alone 173 ± 54 581 ± 91
SBTI 10 154 ± 47 631 ± 102
SBTI 30 194 ± 52 646 ± 100
α1-AT 10 nd 505 ± 105
Trypsin 0.1 294 ± 78 993 ± 185
Trypsin 0.1 + SBTI 10 176 ± 47* 567 ± 110*
Trypsin 0.1 + SBTI 30 173 ± 49* 590 ± 120*
Trypsin 0.1 + α1-AT 10 nd 556 ± 133*
Trypsin 0.3 718 ± 147 1061 ± 366
Trypsin 0.3 + SBTI 10 158 ± 45* 543 ± 122*
Trypsin 0.3 + SBTI 30 256 ± 67* 654 ± 141*
Trypsin 0.3 + α1-AT 10 nd 579 ± 131*
  1. Data shown are mean ± SE for five separate experiments. Trypsin, inhibitor, and trypsin with inhibitor were added to cells for 8 h or 16 h. * P < 0.05 compared with the corresponding concentration of trypsin alone. α1-AT = α1-antitrypsin, SBTI = soybean trypsin inhibitor, nd = not done.