Figure 1
From: Actin polymerisation at the cytoplasmic face of eukaryotic nuclei
Treatment of cells with actin interfering drugs. HeLa cells were treated for 30 min at 37°C with the actin-binding drugs LatA (1μM), CD (1μM) Jaspl (200 nM), or vehicle (DMSO) and then stained with Hoechst 33342. Nuclear shape phenotypes were distinguished ("normal/round" or "irregular" nuclei) and counted by widefield microscopy. (A) The bar graph shows the percentage of normal nuclei (white) versus irregular nuclei (grey) measured from two independent experiments (see methods). (B) The panel shows an example of normal nuclei (after treatment with vehicle) compared with irregular nuclei following drug treatments. Scale bar: 5μm