Living- and single-cell gates and the twelve subfractions. A: Living and single cell-rich gates in FSarea-FSheight (a, blue) and SSarea-SSheight (b, red) are shown. B: SSarea-FSarea profile. Left: dot plot of all cells (black) and cells in the a and b gates (purple dots). Right: contour plot of the cells in the a and b gates. The twelve sorting gates are represented by the green rectangles. C: Schematic representation of the twelve gates. SSarea and FSarea values were normalized to the smallest value and represented as SSn and FSn. D: The number of aggregate cells in the twelve subfractions (n = 120). The SYTO24-stained nuclei in the sorted cells were counted. Shading corresponds to that in C.