Cell size and shape of the sorted cells in the twelve subfractions. A: Phase-contrast micrographs of the sorted cells: spherical and small (a); spherical and large (b); oval (c); irregular surface (d). B-D: The diameter (d
, B), elongation (e, C), and surface roughness (P/A, D) of the cells are shown. These values were evaluated from the phase-contrast images of cells (see Methods). Left, average for each subfraction; middle, average for SSn; right, average for FSn. Shading corresponds to that in Fig. 4C. The number of measured cells is shown on each column in B. The right ordinate of B is the relative cell volume (d
3/d3), where d is the average of d
at (SSn, FSn) = (1.00, 1.24) (arrow head). The relative cell volume at (SSn, FSn) = (2.00, 1.24) (arrow) was about two. Statistically significant for C and D at *P < 0.05; **P < 0.005; ***P < 0.0005. Statistically not significant for B and C at #P > 0.05.