The congression and segregation defects scored in MCAK inhibited cells are displayed. Live imaging of RNAi knockdown and antibody inhibition of P-MCAK show an increase in congression defects, and an increase in segregation defects compared to control RNAi knockdown and IgG injected cells. (A, B) Series of panels from time-lapse phase contrast microscopy of different movies of either control or MCAK inhibited cells that illustrate the different mitotic defects that were scored in all cells. Images chosen are to represent a particular morphological defect and include images taken from both antibody injection experiments and RNAi experiments. (A) Alignment defects and (B) segregation defects. In each panel, the arrow points to the chromosome of interest, and the line indicates the trajectory of chromosome movement and can serve as a reference point for the extent of chromosome movement. Scale bar, 10 μm.