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Table 1 Analysis of the morphological defects and mitotic progression of mitosis in live images of RNAi transfected cells vs. antibody-injected cells.

From: Deciphering protein function during mitosis in PtK cells using RNAi

Measurement Control RNAi MCAK RNAi Control IgG Anti-MCAK
Alignment Defects % cells (n) % cells (n) % cells (n) % cells (n)
Chromosomes lingering at poles 26 (17) 51 (15) 18 (17) 38 (16)
Multiple attempts to congress 33 (17) 49 (15) 18 (17) 56 (16)
Not all chromosomes congress 4 (16) 13 (13) 6 (17) 27 (16)
Loose alignment with high oscillations 13 (16) 89 (16) 24 (17) 87 (16)
Total % cells with alignment defects 39 (18) 88 (16) 41 (17) 88 (17)
Segregation Defects % cells (n) % cells (n) % cells (n) % cells (n)
Straggling chromosomes 11 (17) 53 (12) 25 (16) 50 (16)
Lagging chromosomes 0 (17) 6 (12) 6 (16) 23 (13)
Total % cells with segregation defects 12 (17) 42 (12) 25 (16) 50 (16)
Measurement Control RNAi MCAK RNAi Control IgG Anti-MCAK
Spindle Defects Avg. ± SEM (n) Avg. ± SEM (n) Avg. ± SEM (n) Avg. ± SEM (n)
Spindle length (μm) 15.3 ± 0.5 (14) 12.9 ± 0.5* (12) 14.5 ± 0.6 (16) 12.9 ± 0.4* (17)
Timing of Mitotic Progression Avg. ± SEM (n) Avg. ± SEM (n) Avg. ± SEM (n) Avg. ± SEM (n)
Time between injection and NEB (min) NA NA 5.6 ± 2.2 (17) 2.8 ± 0.8 (18)
Time between NEB and LCC (min) 17.4 ± 1.3 (14) 23.3 ± 7.0 (12) 16.1 ± 1.7 (15) 32.3 ± 3.4* (17)
Time between LCC and Ana A (min) 13.7 ± 2.7 (13) 12.6 ± 1.8 (11) 16.1 ± 1.7 (14) 17.4 ± 2.2 (18)
Total time between NEB and Ana A (min) 30.1 ± 2.7 (17) 32.6 ± 5.9 (14) 33.3 ± 1.7 (17) 47.7 ± 4.1* (18)
  1. NEB, Nuclear envelope breakdown, LCC, the time when the last chromosome begins congressing. NA, not applicable. * Represents a p-value of <0.05 and indicates a significant difference in spindle length or timing.