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Figure 3 | BMC Cell Biology

Figure 3

From: The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization

Figure 3

TBZ affected the polarity of rad26Δ cells. A. Images of untreated and TBZ-treated rad26+ and rad26Δ cells. Cultures of rad26+ (TE236) and rad26Δ (TE257) cells were split and left untreated or treated with 20 μg/ml TBZ for five hours, fixed with paraformaldehyde and observed using DIC. B. Interpretive diagram showing that rad26Δ cells were significantly wider (*) than rad26+ cells. The dimensions of untreated and TBZ-treated cells were quantified using Leica FW4000 image analysis software. The length-to-width (L/W) ratios were calculated and presented here in pictorial representations of rad26+ (outlined in black) and rad26Δ (outlined in white) cells. C. The width of rad26Δ cells increased (+) during TBZ-treatment.

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