TBZ affected the polarity of rad26Δ cells. A. Images of untreated and TBZ-treated rad26+ and rad26Δ cells. Cultures of rad26+ (TE236) and rad26Δ (TE257) cells were split and left untreated or treated with 20 μg/ml TBZ for five hours, fixed with paraformaldehyde and observed using DIC. B. Interpretive diagram showing that rad26Δ cells were significantly wider (*) than rad26+ cells. The dimensions of untreated and TBZ-treated cells were quantified using Leica FW4000 image analysis software. The length-to-width (L/W) ratios were calculated and presented here in pictorial representations of rad26+ (outlined in black) and rad26Δ (outlined in white) cells. C. The width of rad26Δ cells increased (+) during TBZ-treatment.