Loss of rad26+ affected the polar distribution of actin patches but not gross microtubule architecture. A. Gross microtubule architecture was not affected by loss of rad26+. rad26+ (1226) and rad26Δ (1248) cells expressing ectopic atb2-gfp were grown in EMM + 0.2 μM thiamine. TBZ (20 μg/ml) was added to half of each culture for 5 hours, after which cells were fixed with methanol and processed for microscopy. Bar = 4 μm B. rad26+ is required for the polar distribution of actin patches. rad26+ (TE236) and rad26Δ (TE257) cells were grown in YE5S liquid. Half of each culture was treated with 20 μg/ml TBZ for 5 hours before fixing and staining with FITC-Phalloidin (see Methods). Bar = 5 μm C. A diagrammatic representation of polar and non-polar actin patch distributions. D. Graphical representation of data collected from B.