Figure 6

Over expression of rad24 cDNA rescued the TBZ-sensitivity of rad26Δ cells, but not mad2Δ cells. A. Results of the over expression (OE) screen (see Methods). Four cDNAs suppressed the TBZ-sensitivity of rad26Δ cells, and three of these also suppressed the TBZ-sensitivities of nda2KM52 and mad2Δ cells. B. Only OErad24⁺ specifically suppressed the growth of rad26Δ cells on TBZ. Wild type (TE236), rad26Δ (TE257) and mad2Δ (TW1219) were transformed with a plasmid containing inducible rad24 cDNA (pTW909) and streaked onto EMM and EMM + TBZ medium containing the vital stain Phloxin B. Pictures were taken after 3 days of growth at 30°C. Upper left = EMM; Upper right = EMM + TBZ (20 μg/ml) C. rad24Δ cells were sensitive to TBZ. Wild type (TE236), rad26Δ (TE257), rad24Δ (TE465), rad25Δ (TE464) and chk1Δ cds1Δ (TE892) were streaked onto YE5S (top) and YE5S + 20 μg/ml TBZ (bottom) and incubated at 30°C for three days.