Table 1 Fission yeast plasmids and strains

pTE169

nmt-rad26 (cDNA) Leu⁺

al-Khodairy et al., 1994

pTE102

nmt (empty vector) Leu⁺

Maundrell, 1993

pTW909

nmt-rad24 (full length cDNA isolated from over expression screen, Figure 8) Leu⁺

This study

TE236

leul-32 ura 4-d18 h ^-

Kostrub et al., 1998

TE257

rad26::ura4 ⁺ ade6-704 leul-32 ura4-D18 h ^-

Al-Khodairy et al. (1994)

TE369

nda2-KM52 leu1-32 h ⁺

Toda et al., 1983

TE464

rad25::ura4 ⁺ ade6-704 leu1-32 ura4-D18 h ⁺

Ford et al., 1994

TE465

rad24::ura4 ⁺ ade6-704 leu1-32 ura4-D18 h ⁺

Ford et al., 1994

TE550

kin1::LEU2 ade6-704 leu1-32 ura4-294 h90

Levin and Bishop, 1990

TE570

rad3::ura4 ⁺ ade6-704 leu1-32 ura4-D18 h ^-

Bentley et al., 1996

TE583

cdc25-22 h ^-

Nurse et al., 1976

TE787

rad3::ura4 ⁺ ade6-M210 [Ch16 ade6-216]

Gift of CR Chapman

TE892

chk1::ura4 ⁺ cds1::ura4 ⁺ ura4-D18 leu1-32

Gift of C. Kostrub

TE1197

rad26::rad26-gfp (G418 ^R ) leu1-32 ura4-D18 h ^-

TW1207

leu1^-, ura^-, Cen1-GFP [dis1 promoter 5'-lacI-gfp] (at his7 locus) lacO repeat (at lys1 locus which is 30 Kb from Cen1) h⁺

Nabeshima et al., 1998

TW1219

mad2::ura4 ura4-D18 leu1-32 h ^-

Sugimoto et al., 2004

TW1222

[Ch16 ade6-216] ade6-210 leu1-32 ura4-D18

Javerzat et al., 1996

TW1224

rad26::ura4 ⁺ ade6-210 ura4-D18 [Ch 16 ade6-216]

This study

TW1226

leu1-32 pDQ105 (LEU⁺ nmt-atb2-gfp) h^-

Ding et al., 1998

TW1235

rad26::ura4 ⁺ rad24::ura4 ⁺

This study

TW1237

rad25::ura4⁺ rad26::rad26-gfp (G418^R)

This study

TW1238

rad24::ura4⁺ rad26::rad26-gfp (G418^R)

This study

TW1239

rad22::rad22-gfp (kan^r) ade6-210 leu1-32 h^-

Gift of Miguel Ferrerira

TW1248

rad26::ura4⁺ ura4-D18 ade6-708 pDQ105 (LEU⁺ nmt-atb2-gfp) h^-

This study

TW1261

cdc25-22 Cen1-GFP

This study

TW1262

cdc25-22 rad26::ura4⁺ Cen1-GFP

This study