Figure 7

Relative abundance of NETs in isolated nuclear envelopes and microsomal membranes of liver. Mouse liver NE and MM fractions were analyzed by immunoblotting with antibodies against NET25, NET32, and NET37 as indicated above each image. Control blots with anti-emerin and anti-calnexin antibodies, as well as a representative Ponceau Red stain of one of the membranes used in these experiments are shown on the right. Positions of the target proteins are indicated by black arrowheads. Labeling of these bands was strongly diminished by pre-incubating the antibodies with the cognate peptides (data not shown), whereas labeling of nonspecific bands was not. A nonspecific in the microsomal lane of the anti-NET37 blot is indicated by an asterisk. Where feasible, signal intensities normalized to the signal in the NE lanes are shown below each membrane.