Figure 2

Time course of NO production and iNOS expression in LPS stimulated RAW264.7 macrophages incubated with CEES. Panel A: Macrophages were incubated with 10 ng/ml LPS alone, 300 μM CEES alone or simultaneously with both 300 μM CEES 10 ng/ml LPS for various time intervals (as indicated). NO production measured as concentration of nitrite in culture medium. Panel B: Western blot analysis of iNOS protein from the cells incubated with 300 μM CEES with or without 10 ng/ml LPS; cell lysates were prepared after 3, 6, 12, or 24 hour incubation (as indicated) as described in Materials and Methods; L, LPS; C, CEES.