Figure 3
Actin binding of Talin-a and Talin-b I/LWEQ modules. Actin binding curves for the interaction of GST-Talin-a.2341–2531 and GST-Talin-b.2341–2531 with F-actin were calculated with GraphPad Prism 4.0 as previously described [22]. The Talin-b I/LWEQ module bound to F-actin with a 4-fold higher affinity than that of Talin-a (Kd = 0.19 μM vs. 0.76 μM). Each data point is the average of three independent measurements.