Figure 7

Role of Cdk-5 in the regulation of (A) Humbug and (B) Junctin expression. SH-Sy5y cells were transfected with recombinant plasmids to over-express Luciferase (pLuc) as a negative control, Cdk-5, p25, p35, Cdk-5+p25, or Cdk-5+p35 as indicated in the box below the graphs. The cells were harvested 48 hours later to measure gene expression by real time quantitative RT-PCR (see Methods). The graphs depict the mean ± S.D. of Humbug, or Junction expression with values normalized to 18S rRNA measured in the same samples. Statistical comparisons were made using ANOVA with post hoc Fisher LSD tests (*P < 0.001 relative to pLuc-transfected control cells).