|
Primer*
|
Sequence (5'→3')
|
Amplicon Size (bp)
|
|---|
|
18 S For
|
GGACCAGAGCGAAAGCATTTGCC
| |
|
18 S Rev
|
TCAATCTCGGGTGGCTGAACGC
|
50
|
|
AAH For
|
GGGAGATTTTATTTCCACCTGGG
| |
|
AAH Rev
|
CCTTTGGCTTTATCCATCACTGC
|
256
|
|
Humbug For
|
GCTGGGTTGATTGAGGATGTGTG
| |
|
Humbug Rev
|
GCAGGGGGAAAAAGTCACCTTATC
|
301
|
|
Junctin For
|
CCTGAGTCAAGGAAGGAAAGTAAG
| |
|
Junctin Rev
|
GCCGTTTCTTTTCTGGGTATTTCC
|
308
|
|
IRS-1 For
|
TGCTGGGGGTTTGGAGAATG
| |
|
IRS-1 Rev
|
GGCACTGTTTGAAGTCCTTGACC
|
68
|
|
IRS-2 For
|
AAAATTGGCGGAGCAAGGC
| |
|
IRS-2 Rev
|
ATGTTCAGGCAGCAGTCGAGAG
|
64
|
|
IRS-4 For
|
CCGACACCTCATTGCTCTTTTC
| |
|
IRS-4 Rev
|
TTTCCTGCTCCGACTCGTTCTC
|
74
|
- *For-forward; Rev-reverse; AAH = aspartyl (asparaginyl)-β-hydroxylase; IRS = insulin receptor substrate
