Growth Factor | IRS-1/18S × 10-6 | IRS-2/18S × 10-6 | IRS-4/18S × 10-6 |
|---|
Control | 7.18 ± 0.88*, + | 1.19 ± 0.21 | 1.37 ± 0.27 |
Insulin | 18.66 ± 2.21*, ++ | 2.18 ± 0.44 + | 3.96 ± 0.61 + |
IGF-1 | 32.61 ± 2.55*, ++ | 1.37 ± 0.24 | 2.01 ± 0.21 + |
- SH-Sy5y cells were stimulated with vehicle (Control), 50 nM insulin, or 25 nM IGF-1 for 24 hours, then analyzed by real time quantitative RT-PCR to measure mRNA levels of insulin receptor substrate (IRS) subtypes 1, 2, and 4 using the primer pairs listed in Table 2. The results were normalized to 18S ribosomal RNA measured in the same samples. The values listed represent mean ± S.D. of results obtained from 6 replicate cultures. *P < 0.001 relative to IRS-2 and IRS-4 measured in the same cultures. ++ P < 0.001; + P < 0.05 relative to control.
