Figure 3

CRAC-GFP (A/B), PI3K2-CFP (C/D), ABP120-GFP (E/F) and Actin-GFP (G/H) can translocate in response to a micropipette filled with 1 μM cAMP in the presence of cycloheximide. Polarisation of these markers was followed in the absence (A, C, E, G) or presence (B, D, F, H) of 2 mM cycloheximide. The position of the cAMP-filled micropipette is indicated by the white "<" symbol. The micropipette always comes in from the right-hand side. Fluorescent images were taken as sections using a 60× objective lens. Scale bar represents 10 μm. For movies, see Additional files.