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Table 1 Percentage of apoptotic cells: comparison of results obtained with different methods in HT 1376 cells

From: Sequential events of apoptosis involving docetaxel, a microtubule-interfering agent: A cytometric study

UNTREATED CULTURES
METHOD CULTURE TIME
  6 hrs 16 hrs 24 hrs 48 hrs 72 hrs 96 hrs 120 hrs
Cell Cycle HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M
(PI staining) 1.0 42.6 37.4 20.0 1.0 44.0 35.4 20.6 2.0 47.9 30.5 21.6 2.6 60.5 31.0 8.5 2.1 64.5 25.7 9.8 3.3 70.1 19.3 10.6 5.6 78.1 12.3 9.6
  6 hrs 16 hrs 24 hrs 48 hrs 72 hrs 96 hrs     
JC-1 1.5 2.0 3.5 2.5 4.6 5.1     
Low FSC area 4.4 5.6 8.9 11.8 10.8 30.7     
Low FSC/SSC area 1.7 0.4 0.8 2.3 3.0 4.5     
MAA+PI staining E L N   E L N   E L N   E L N   E L N   E L N      
  N.E. N.E. N.E.   N.E. N.E. N.E.   0.5 0.6 5.4   1.5 1.3 7.2   3.1 3.4 10.6   5.0 5.8 10.8      
AnnV+PI staining E L N   E L N   E L N   E L N   E L N   E L N      
  N.E. N.E. N.E.   N.E. N.E. N.E.   2.9 0.7 4.5   2.0 3.2 5.1   1.2 4.7 7.3   3.1 5.9 8.2      
TUNEL N.E. N.E. 0.5 0.8 1.6 1.5     
DOCETAXEL-TREATED CULTURES
METHOD CULTURE TIME
  6 hrs 16 hrs 24 hrs 48 hrs 72 hrs 96 hrs 120 hrs
Cell Cycle HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M HP G0/1 S G2/M
(PI staining) 3.1 30.9 27.5 41.6 4.9 25.7 29.0 45.3 5.5 3.1 9.1 87.8 35.5 5.9 12.3 81.8 33.3 19.8 7.9 72.3 39.0 31.4 10.9 57.7 53.5 57.1 23.4 19.5
  6 hrs 16 hrs 24 hrs 48 hrs 72 hrs 96 hrs     
JC-1 3.0 10.4 24.5 53.6 60.0 91.3     
Low FSC area 5.4 7.4 6.6 2.2 2.3 3.3     
Low FSC/SSC area 6.5 8.5 24.2 29.6 32.5 33.6     
MAA+PI staining E L N   E L N   E L N   E L N   E L N   E L N      
  N.E. N.E. N.E.   N.E. N.E. N.E.   0.5 0.7 9.9   28.1 25.1 12.5   19.0 39.6 12.9   21.2 42.7 15.6      
AnnV+PI staining E L N   E L N   E L N   E L N   E L N   E L N      
  N.E. N.E. N.E.   N.E. N.E. N.E.   6.5 0.9 5.1   14.7 15.9 7.5   27.7 43.4 8.3   21.7 56.5 9.9      
TUNEL N.E. N.E. 4.7 9.3 28.6 70.4     
  1. Top table: untreated (control) HT1376 bladder carcinoma cultures. Bottom table: HT1376 bladder carcinoma cultures were treated for 1 hour with 3 μg/mL docetaxel, after which the drug was washed out and the cells were further cultured. Figures are percentages of cells with the indicated characteristics. Data are the average of 3 independent experiments, with errors below 10%. For details, see the Methods section. N.E. = Not Examined; HP = Hypodiploid Peak; JC-1 = cells undergoing Δψm transition; E = early apoptosis, i.e. MAA+/PI- or AnnV+/PI-; L = late apoptosis, i.e. MAA+/PI+ or AnnV+/PI+; N = necrosis, i.e. MAA-/PI+ or AnnV-/PI+