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Table 2 Percentage of apoptotic cells: comparison of results obtained with different methods in the U937 myelomonocytic cell line

From: Sequential events of apoptosis involving docetaxel, a microtubule-interfering agent: A cytometric study

Method Culture time
  16 hrs 24 hrs 48 hrs Untreated controls, 48 hrs
JC-1 15.6 27.3 56.4 4.0
MAA + PI staining E L N E L N E L N E L N
  18.4 2.0 4.6 23.8 4.4 8.4 38.0 29.0 6.5 1.0 1.5 5.6
Ann V + PI staining E L N E L N E L N E L N
  19.5 6.5 1.2 28.7 10.1 3.5 40.9 22.0 2.8 2.9 1.5 1.8
  1. U937 myelomonocytic cells were treated for 1 hour with 3 μg/mL docetaxel, after which the drug was washed out and cells were further cultured. Data are the average of 2 experiments, with nearly identical results. For details, see the Methods section.
  2. JC-1 = cells undergoing Δψm transition; E = early apoptosis, i.e. MAA+/PI- or AnnV+/PI-; L = late apoptosis, i.e. MAA+/PI+ or AnnV+/PI+; N = necrosis, i.e. MAA-/PI+ or AnnV-/PI+