Skip to main content

Table 3 Effect of pre-incubation with CsA on DOC-treated HT 1376 cells.

From: Sequential events of apoptosis involving docetaxel, a microtubule-interfering agent: A cytometric study

  1 h 16 hrs 24 hrs
Control
JC-1 1.0 2.1 4.7
AnnV + PI staining E L N E L N E L N
  1.9 0.5 1.5 2.1 0.9 3.1 2.8 1.1 5.0
DOC-treated
JC-1 1.0 9.5 24.0
AnnV + PI staining E L N E L N E L N
  2.1 0.9 2.0 5.0 0.5 3.5 6.0 2.5 5.5
CsA + DOC treated
JC-1 1.0 5.2 23.1
AnnV + PI staining E L N E L N E L N
  2.0 0.7 2.5 5.0 1.1 4.2 5.0 4.3 8.4
  1. HT 1376 bladder carcinoma cells were pre-incubated for 30 min with 1 μM CsA, after which 3 μg/mL docetaxel was added. After a 1-h treatment, both drugs were washed out and cells were further cultured. Data are percentages of cells with the indicated staining characteristics. For details, see the Methods section.
  2. JC-1 = cells undergoing Δψm transition; E = early apoptosis, i.e. AnnV+/PI-; L = late apoptosis, i.e. AnnV+/PI+; N = necrosis, i.e. AnnV-/PI+