| 1 h | 16 hrs | 24 hrs |
---|
Control
|
JC-1 | 1.0 | 2.1 | 4.7 |
AnnV + PI staining | E | L | N | E | L | N | E | L | N |
 | 1.9 | 0.5 | 1.5 | 2.1 | 0.9 | 3.1 | 2.8 | 1.1 | 5.0 |
DOC-treated
|
JC-1 | 1.0 | 9.5 | 24.0 |
AnnV + PI staining | E | L | N | E | L | N | E | L | N |
 | 2.1 | 0.9 | 2.0 | 5.0 | 0.5 | 3.5 | 6.0 | 2.5 | 5.5 |
CsA + DOC treated
|
JC-1 | 1.0 | 5.2 | 23.1 |
AnnV + PI staining | E | L | N | E | L | N | E | L | N |
 | 2.0 | 0.7 | 2.5 | 5.0 | 1.1 | 4.2 | 5.0 | 4.3 | 8.4 |
- HT 1376 bladder carcinoma cells were pre-incubated for 30 min with 1 μM CsA, after which 3 μg/mL docetaxel was added. After a 1-h treatment, both drugs were washed out and cells were further cultured. Data are percentages of cells with the indicated staining characteristics. For details, see the Methods section.
- JC-1 = cells undergoing Δψm transition; E = early apoptosis, i.e. AnnV+/PI-; L = late apoptosis, i.e. AnnV+/PI+; N = necrosis, i.e. AnnV-/PI+