Figure 4

CTGF is involved in high glucose-induced proliferation of cultured HUVSMCs. Quiescent cells were transfected with scrambled or CTGF-siRNA expression plasmids for 24 hours and then exposed to HG for 48 hours followed by the assessment of [³H]-thymidine incorporation (a) and cell number counting (b). Each value is the mean ± SEM of 6 separate experiments. * P < 0.05 vs scrambled siRNA transfection under normal glucose (NG) condition. # P < 0.05 vs scrambled siRNA transfection under high glucose (HG) condition. Scrambled siRNA: scrambled siRNA plasmid transfection; siRNA: CTGF-siRNA plasmid transfection.