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Figure 3 | BMC Cell Biology

Figure 3

From: Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2

Figure 3

Stimulation of Tie-2 phosphorylation in bmECs by Ang1 and Ang2. A/ Stimulation of bmLECs, bmVECs, and bmAECs with 800 ng/mL Ang1 (clustered with anti-His antibody), or 800 ng/mL Ang2, or with clustering antibody alone (Mock). Ang1 stimulation resulted in activation of Tie-2 in bmLECs and to a lesser extent bmAECs and bmVECs. B/ Phosphorylated Tie-2 signals from blots of three independent experiments were measured via densitometry (ImageQuant) and normalized to corresponding total Tie-2 levels. Paired Ang1- and Ang2- stimulated, normalized Tie-2 phosphorylation levels in each experiment were compared by ttest and p-values for 95% CI are shown. Both Ang1 and Ang2 stimulated Tie-2 to comparable levels in bmLECs, whereas Ang1 was more effective in stimulating Tie-2 in bmVECs and bmAECs. C/ Phosphorylated ERK1/2 signals from blots of the same three independent experiments from B/ were measured via densitometry (ImageQuant) and normalized to corresponding total ERK1/2 levels. Paired Ang1- and Ang2-stimulated, normalized ERK1/2 phosphorylation levels in each experiment were compared by ttest and p-values for 95% CI are shown. Ang1 was more effective in stimulating ERK1/2 phosphorylation in all three cell types than Ang2. D/ Stimulation of bmLECs with 800 ng/mL Ang2 resulted in phosphorylation of tyrosine residue 992 on Tie-2.

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