Subcellular localization of full length FLAG-tagged NCOA7 protein in human MCF-7 cells. MCF-7 cells were cultured in hormone-free medium and transiently transfected with FLAG-tagged full-length NCOA7. Two days after transfection, cells were treated without or with 100 nM E2 for 2 hours. Cellular localization of NCOA7 was detected by immunofluorescence using an anti-FLAG antibody (red stain). Cell nuclei were indicated by the blue DAPI stain. -E2, no estrogen, +E2, estrogen treated cells.