Protein expression of NCOA7 after treatment with hydrogen peroxide. MCF-7 cells were treated with indicated concentrations of hydrogen peroxide (H2O2) for 1, 4, 8, or 16 hours. Whole cell lysates were prepared for western analysis. The protein band corresponding to NCOA7 was indicated by its loss after siRNA-mediated inhibition. Calnexin is a loading control.