Figure 5

Effect of AG490 (AG), PD98059 (PD) and LY294002 (LY) on MMP-2 and MMP-9 activity release and MMP-9 mRNA expression in HDFs. (A) HDFs were incubated with AG490 (40 μM), PD98059 (50 μM) and LY294002 (40 μM) in the presence or absence of thrombin (Th, 5 U/ml) at 37°C for 2 h and 6 h, and MMP-2 and MMP-9 activities were detected with zymograph. (B) HDFs were incubated with AG490, PD98059 and LY294002 in the presence or absence of Th at 37°C for 6 h, and MMP-9 mRNA was detected by RT-PCR analysis. The relative levels of MMP-9 mRNA were expressed as the ratio to β-actin. The values shown are Mean ± SD for four separate experiments. Cells from each one of the four dermal fibroblast donors were used for one independent experiment. ^† P < 0.05 compared with the response to medium alone (Con); * P < 0.05 compared with the response to thrombin alone.