The N-terminus and alpha-5, alpha-6 helices of the pro-apoptotic protein Bax, modulate functional interactions with the anti-apoptotic protein Bcl-xL

Table 1 Percent viability with tissue specific GAL4 drivers

	vg- GAL4	twist- GAL4	scalloped- GAL4	eyeless- GAL4	collagen- GAL4
*UAS-Bax-GFP*	6.8	7.6	0	0	0
(BA64III)	(36/527)	(46/602)	(0/141)	(0/96)	(0/130)
*UAS-Bax30–192-GFP*	8.9	0.6	1.6	0	0
(DBA8II)	(55/616)	(2/315)	(1/59)	(0/120)	(0/137)
*UAS-Bcl-2*	90.4	104	97.5
(BC57I)	(377/417)	(259/248)	(39/40)

UAS transgenic lines for Bax-GFP (BA64III) or Bax 30–192-GFP (DBA8II) or Bcl-2 (BC57I) were screened for lethality using various tissue specific GAL4 lines as indicated. Percent viability was calculated by assessing the number of Bax-GFP or Bax 30–192-GFP or Bcl-2 expressing flies compared to the appropriate sibling control that eclosed at 25°C. Numbers in parenthesis indicate animals screened for analysis.

ISSN: 2661-8850