Figure 3

Anti-cytotoxicity effect of roxithromycin on SM-treated SAE and BTE cells. Cell viability was examined by Calcein AM/EthD-1 fluorescence staining 24 h after exposure. The images for SAE cells (A) were captured by fluorescence microscopy whereas those for BTE cells (B) by confocal fluorescence microscopy. a) control (unexposed), b) 100 μM roxithromycin, c) 100 μM SM, and d) 100 μM SM + 100 μM roxithromycin. Cells stained green were alive and those stained red dead.