Figure 2

The phenotypes of actin mutants expressed in C2C12cells differed before and after differentiation. A) Percentages of cells with good integration of the expressed EGFP-tagged actin construct (category 1 cells, see Table 1). Most of the mutant actin isoforms showed better incorporation into the actin cytoskeleton in differentiated myotubes than in undifferentiated cells. B) Distribution of different phenotypes of the expressed EGFP-tagged actin constructs in affected C2C12 cells before and after differentiation (category 2 cells, see Table 1). Left bars represent undifferentiated myoblasts, right bars differentiated myotubes. The bars are subdivided, showing the different types of phenotypes that were observed. In some cells, more than 1 phenotype was observed, resulting in total percentages of more than 100%.