Figure 3
From: Phenotypes of Myopathy-related Actin Mutants in differentiated C2C12 Myotubes
Expression of wild-type actin-EGFP. Wild-type muscle actin with a C-terminal EGFP-tag was expressed in undifferentiated C2C12 myoblasts (A) or myotubes that have been differentiated for 4–6 days (B, C). Expressed actin_EGFP co-localized with endogenous filamentous actin, as visualized with phalloidin in myoblasts (A, A") and myotubes (B, B"). In a subset of myotubes, a striated staining-pattern for alpha-actinin revealed sarcomeric structures, with expressed EGFP-actin co-localizing within the sarcomeric thin-filament (C, C"; inset). (Scale bars: 5 μm)