Figure 7
From: Phenotypes of Myopathy-related Actin Mutants in differentiated C2C12 Myotubes
Expression of the AM-linked G15R-actin mutant. Expression of the EGFP-tagged G15R-actin isoform in myoblasts produced small cytoplasmic aggregates in many cells (A; long arrow), co-staining with phalloidin but not with alpha-actinin (A', A"; long arrows). In differentiated myotubes, the mutant actin gets integrated, but produced extremely thickened stress fibres (B, B"; long arrows). Sometimes this resulted in big accumulations of polymerized actin that co-stained with phalloidin (C, C"; long arrows).