Figure 3

p53 and p21^WAF1 expression and doxorubicin-resistant cell viability of primary and immortalized MPF and PF cells. A. Expression of p53 and p21^WAF1 proteins in the primary and immortal MPF and PF cells. α-tubulin was used as a loading control. B. Expression of p53 and p21^WAF1 proteins in the primary and immortal MPF and PF cells grown in the absence or presence of doxorubicin (3 uM). α-tubulin was used as a loading control. C. Cell viability (%) of the primary and immortal MPF and PF cells grown in the absence or presence of doxorubicin (1, 3, and 5 uM) for 24 hr. The a, b, c, and d indicate significant differences (p < 0.05).