Figure 4

Anchorage-independent growth and chromosome abnormalities of the immortal MPF cells. A. Representative photographs showing primary and immortal MPF and PF cells grown under soft-agar culture conditions for 3 weeks (Left), and the foci numbers of primary and immortal MPF and PF cells grown in the soft-agar (Right). HeLa cells served as the positive control. The number shown in the graph are means ± SEM (n = 3). Asterisk (*) indicates a significant difference (p < 0.001). B. Representative photographs showing metaphase chromosomes of primary and immortal MPF and PF cells (400× magnifications; Left), and average number of chromosomes of primary and immortal MPF and PF cells (Right). The number shown in the graph are means ± SEM (n = 100). Asterisk (*) indicates a significant difference (p < 0.05).