Figure 2

Intracellular localization of wild type and glycosylation site mutated PPT1 in HeLa cells. HeLa cells were transfected with the wild type pCMV5-PPT1 plasmid or with plasmids carrying mutations S199A, S214A, S214A and S199A + S214A. The cells were double-stained using the polyclonal antibody for PPT1 (red) and the monoclonal antibody for LAMP-1, lysosomal membrane protein (green). Colocalization is shown in yellow.