Generation of gxcDD- cells. (A) Schematic representation of the strategy to generate gxcDD- cells. (B) Southern blot analysis of StuI and BciVI digested genomic DNA to confirm the recombination event in gxcDD- cells. Two independent clones were analysed. (C) Western blot analysis indicates the complete loss of GxcDD protein in gxcDD- cells (upper panel). The blot was reprobed with actin specific mAb act1-7 as a loading control (lower panel).