Figure 8

Streaming defect in gxcDD^- cells. (A) AX2 and gxcDD^- cells were allowed to starve on plastic plates. Images were continuously recorded. Streams formed in gxcDD^- cells broke down to aggregates. (B) Streaming was also recorded in wild type and gxcDD^- cells using an exogenous source of cAMP in a capillary chemotaxis assay. gxcDD^- cells did migrate towards the cAMP source but did not form streams. (C) gxcDD^- and AX2 cells were allowed to starve in Soerensen buffer at a density of 1 × 10⁷ cells/ml and samples were withdrawn at the indicated times. The percentage of aggregated cells was determined by measuring the OD₆₀₀. The data represent the average of three independent experiments.