Correlation between the maximal Ca2+ mobilization and DMR responses induced by PAR agonists. (a) The DMR signals induced by PAR agonists: TFRGAP (20 μM), GYPGQV (20 μM), SLIGRL-amide (20 μM), SLIGKV-amide (20 μM), thrombin (40 unit/ml), SFLLR-amide (20 μM), trypsin (1024 nM), SFLLR-amide+SLIGRL-amide (each at 20 μM). (b) Comparison of the maximal DMR and Ca2+ mobilization responses induced by different PAR agonists. The DMR response was calculated using the amplitude of the P-DMR event. Since trypsin at doses greater than ~1000 nM led to significant cell detachment (ref. 16), the DMR signal induced by trypsin at 1024 nM was used as its maximal response.