Figure 6
From: Optical biosensor differentiates signaling of endogenous PAR1 and PAR2 in A431 cells
Cholesterol removal impairs the agonist-induced Ca2+ mobilization. mβCD was used to deplete cell surface cholesterol. Its effect on agonist-induced Ca2+ mobilization was examined. The agonists were trypsin (200 nM) and thrombin (40 unit/ml).