Figure 7
From: Optical biosensor differentiates signaling of endogenous PAR1 and PAR2 in A431 cells
Cholesterol removal attenuates the agonist-induced DMR signals. mβCD was used to deplete cell surface cholesterol. Its effect on the amplitudes of the P-DMR events induced by (a) thrombin (40 unit/ml) or (b) trypsin (200 nM) was analyzed. In comparison, the effect of αCD was also included. (c) The DMR signals of A431 cells induced by A23187 without or with the pre-treatment with 5 mM mβCD.