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Table 1 EC50 values of PAR agonists in A431. EC50 values were obtained using conventional Ca2+ flux assay, in comparison with those obtained using the MRCAT. In the case of MRCAT data, the amplitudes of both P-DMR and N-DMR events as a function of agonist concentration (as indicated in Fig. 1c) were used to calculate EC50.

From: Optical biosensor differentiates signaling of endogenous PAR1 and PAR2 in A431 cells

Ligand EC50(n = 3)
  Ca2+flux assay P-DMR N-DMR
Trypsin 45.7 ± 5.8 nM 98.0 ± 27.8 nM 102.1 ± 21.9 nM
SLIGRL-amide 2.5 ± 0.3 μM 2.3 ± 0.6 μM 3.2 ± 0.8 μM
SLIGKV-amide 3.8 ± 0.4 μM 6.1 ± 1.0 μM 9.1 ± 2.2 μM
Thrombin 6.0 ± 1.0 unit/ml 9.6 ± 2.0 unit/ml 11.0 ± 1.9 unit/ml
SFLLR-amide 5.0 ± 0.4 μM 1.9 ± 0.1 μM 3.1 ± 0.2 μM