Figure 1

Smad4, Smad7 and SARA co-localize on small cytoplasmic vesicles with the early endosomal marker EEA1 in E7 chicken lenses. A. Diagram of the organization of the lens. B. EEA1 (green), Smad4 (red). D. EEA1 (green), Smad7 (red). F. EEA1 (green), SARA (red). C, E and G are higher magnification images of the regions outlined in figures B, D and F respectively. The insets in C, E and G show 2X magnified images of antibody stained cytoplasmic vesicles (LE – lens epithelia, LF – lens fibers)