Localization of Smad4 and pSmad1/5/8 on early endosomes in E7 chicken lenses. A. EEA1 (green), Smad4 (red) and TOPRO (blue). C. EEA1 (green), pSmad1/5/8 (red) and TOPRO (blue). B and D are higher magnification images of the regions outlined in figures A and C respectively. The insets in B and D show 2X higher magnification images of antibody-stained cytoplasmic vesicles. Both Smad4 and pSmad1/5/8 show co-localization (yellow) with EEA1.