Figure 3
From: Absence of gemin5 from SMN complexes in nuclear Cajal bodies
In Hela PV cells, which have separate gems and CBs, gemin5 colocalizes with gems, not CBs, in those rare cells that do have gemin5-positive nuclear bodies. HeLa strain PV cells grown on coverslips were fixed with 1% formalin in PBS and permeabilized with 1% Triton X-100. (A) Separation of gems (red: MANSMA1 mAb and Alexa-546 anti-mouse Ig) and CBs (green: rabbit anti-coilin and FITC anti-rabbit Ig) in HeLa PV, counterstained with DAPI (blue). Rare gemin5-positive nuclear bodies were double-labelled with (B) mAb GEM5P and Alexa-546 anti-mouse Ig and (C) rabbit anti-SMN and FITC anti-rabbit Ig to show colocalization of SMN and gemin5 (white arrows). (D) is an overlay from a double label with GEM5P (red) and rabbit anti-coilin (green) with a DAPI counterstain (blue), showing no colocalization.