Figure 5

All gemins, except gemin5, are proportionately reduced in SMN-deficient human skin fibroblasts. Total SDS extracts of the two skin fibroblast cell lines (Coriell cell lines, GM08333, control and GM03813, SMA) were subjected to SDS-PAGE on adjacent lanes of a 7% or 14% acrylamide gel (cf. Fig. 1), followed by western blotting with the same antibodies used in Fig. 2 plus MANSIP1A for gemin2. β-actin was used as a control for equal loading of the gel lanes. Microdensitometry was used to express protein levels in SMA fibroblasts as a percentage of those in control fibroblasts.