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Figure 8 | BMC Cell Biology

Figure 8

From: Absence of gemin5 from SMN complexes in nuclear Cajal bodies

Figure 8

Distribution of SMN and gemins between large, fast-sedimenting SMN complexes and slower sedimenting fractions. HeLa total, cytoplasmic and nuclear extracts were prepared and analyzed by centrifugation on 15–30% sucrose density gradients. Thirty 1 ml fractions were collected from each gradient and the protein contents were concentrated into 0.1 ml using Strataclean resin. Every other fraction (1, 3, 5, etc) was subjected to SDS-PAGE for western blotting using appropriate antibodies. The size markers were immunoglobulin (7S), catalase (11S) and thyroglobulin (19S).

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